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ifu c pneumoniae strain cwl029 atcc vr 1310  (ATCC)


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    ATCC ifu c pneumoniae strain cwl029 atcc vr 1310
    Ifu C Pneumoniae Strain Cwl029 Atcc Vr 1310, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+pneumoniae+atcc+vr+1310/Chlamydia+pneumoniae+Grayston+et+al/pmc05723314-47-9-14
    Average 92 stars, based on 98 article reviews
    ifu c pneumoniae strain cwl029 atcc vr 1310 - by Bioz Stars, 2026-09
    92/100 stars

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    Concentration Assay:

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Conversely, at a concentration of 5 IFU/ml or greater, 9 of 10 replicates would be expected to be positive with either PCR, and a single PCR determination would be positive 90 to 95% of the time. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Probit a C. pneumoniae IFU/ml (95% CI) Non-nested PCR b Nested PCR c 0.01 0.02 (0.01, 0.03) 0.01 (0.003, 0.02) 0.05 0.05 (0.02, 0.08) 0.03 (0.01, 0.05) 0.10 0.08 (0.04, 0.13) 0.05 (0.02, 0.08) 0.15 0.12 (0.07, 0.18) 0.07 (0.04, 0.11) 0.20 0.16 (0.10, 0.24) 0.09 (0.06, 0.14) 0.30 0.27 (0.17, 0.40) 0.15 (0.10, 0.23) 0.40 0.41 (0.28, 0.61) 0.24 (0.16, 0.35) 0.50 0.61 (0.42, 0.92) 0.35 (0.24, 0.52) 0.60 0.92 (0.62, 1.42) 0.53 (0.36, 0.80) 0.70 1.41 (0.94, 2.29) 0.81 (0.55, 1.28) 0.80 2.34 (1.51, 4.08) 1.34 (0.88, 2.23) 0.90 4.69 (2.82, 9.25) 2.70 (1.66, 5.12) 0.95 8.34 (4.68, 18.4) 4.79 (2.77, 10.2) 0.99 24.50 (11.9, 67.8) 14.1 (7.07, 37.3) Open in a separate window a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b Campbell et al. ( 6 ). c Tong and Sillis ( 23 ). ..

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Relationship between the predicted number of PCR replicates needed to achieve various test sensitivities and C. pneumoniae concentrations Probita C. pneumoniae concn(IFU/ml)b No. of replicates needed to achieve sensitivity of c: 50% 80% 90% 95% 0.01 0.01 69 161 230 299 0.05 0.03 14 32 45 59 0.10 0.05 7 16 22 29 0.20 0.09 4 8 11 14 0.30 0.15 2 5 7 9 0.40 0.24 2 4 5 6 0.50 0.35 1 3 4 5 0.60 0.53 1 2 3 4 0.70 0.81 1 2 2 3 0.80 1.34 1 1 2 2 0.90 2.70 1 1 1 2 0.95 4.79 1 1 1 1 0.99 14.10 1 1 1 1 a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b C. pneumoniae ATCC VR-1310 concentration as calculated from probit analysis. c Replicates calculated in Microsoft Excel spreadsheet from the following equation: probability (at least 1 positive in n replicates) 5 1 2 (1 2 probit)n. on M arch 15, 2015 by S U N Y H S C B M E D IC A L R E S E A R C H LIB R A R Y http://jcm .asm .org/ D ow nloaded from at lower concentrations of target DNA, and at these concentrations replicates of 3, 5, or 10 would identify increasingly more specimens as positive compared with a single PCR. ..

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS). .. The SPSS statistical program generated the probit (predicted proportion positive) versus the C. pneumoniae concentration with 95% confidence intervals (CI) shown in Table .

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Conversely, at >5 IFU/ml, a single PCR determination is 95% sensitive for detecting C. pneumoniae , and replicates would not increase sensitivity further. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Probit a C. pneumoniae concn (IFU/ml) b No. of replicates needed to achieve sensitivity of c : 50% 80% 90% 95% 0.01 0.01 69 161 230 299 0.05 0.03 14 32 45 59 0.10 0.05 7 16 22 29 0.20 0.09 4 8 11 14 0.30 0.15 2 5 7 9 0.40 0.24 2 4 5 6 0.50 0.35 1 3 4 5 0.60 0.53 1 2 3 4 0.70 0.81 1 2 2 3 0.80 1.34 1 1 2 2 0.90 2.70 1 1 1 2 0.95 4.79 1 1 1 1 0.99 14.10 1 1 1 1 Open in a separate window a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b C. pneumoniae ATCC VR-1310 concentration as calculated from probit analysis. c Replicates calculated in Microsoft Excel spreadsheet from the following equation: probability (at least 1 positive in n replicates) = 1 − (1 − probit) n . Relationship between the predicted number of PCR replicates needed to achieve various test sensitivities and C. pneumoniae concentrations In Fig. , the relationship between increasing number of replicates and overall test sensitivity is presented in graphical form, with the curve for the 10 replicate PCR curve on the far left and that for a single PCR on the far right. ..

    Polymerase Chain Reaction:

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Conversely, at a concentration of 5 IFU/ml or greater, 9 of 10 replicates would be expected to be positive with either PCR, and a single PCR determination would be positive 90 to 95% of the time. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Probit a C. pneumoniae IFU/ml (95% CI) Non-nested PCR b Nested PCR c 0.01 0.02 (0.01, 0.03) 0.01 (0.003, 0.02) 0.05 0.05 (0.02, 0.08) 0.03 (0.01, 0.05) 0.10 0.08 (0.04, 0.13) 0.05 (0.02, 0.08) 0.15 0.12 (0.07, 0.18) 0.07 (0.04, 0.11) 0.20 0.16 (0.10, 0.24) 0.09 (0.06, 0.14) 0.30 0.27 (0.17, 0.40) 0.15 (0.10, 0.23) 0.40 0.41 (0.28, 0.61) 0.24 (0.16, 0.35) 0.50 0.61 (0.42, 0.92) 0.35 (0.24, 0.52) 0.60 0.92 (0.62, 1.42) 0.53 (0.36, 0.80) 0.70 1.41 (0.94, 2.29) 0.81 (0.55, 1.28) 0.80 2.34 (1.51, 4.08) 1.34 (0.88, 2.23) 0.90 4.69 (2.82, 9.25) 2.70 (1.66, 5.12) 0.95 8.34 (4.68, 18.4) 4.79 (2.77, 10.2) 0.99 24.50 (11.9, 67.8) 14.1 (7.07, 37.3) Open in a separate window a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b Campbell et al. ( 6 ). c Tong and Sillis ( 23 ). ..

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Single PCR curves obtained from probit regression analysis of 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see text) are also shown. .. The remaining four curves were calculated in the spreadsheet program Microsoft Excel using the following formula: probability (at least 1 positive in n replicates) 5 1 2 (1 2 probit)n. a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b Campbell et al. (6). c Tong and Sillis (23).

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Relationship between the predicted number of PCR replicates needed to achieve various test sensitivities and C. pneumoniae concentrations Probita C. pneumoniae concn(IFU/ml)b No. of replicates needed to achieve sensitivity of c: 50% 80% 90% 95% 0.01 0.01 69 161 230 299 0.05 0.03 14 32 45 59 0.10 0.05 7 16 22 29 0.20 0.09 4 8 11 14 0.30 0.15 2 5 7 9 0.40 0.24 2 4 5 6 0.50 0.35 1 3 4 5 0.60 0.53 1 2 3 4 0.70 0.81 1 2 2 3 0.80 1.34 1 1 2 2 0.90 2.70 1 1 1 2 0.95 4.79 1 1 1 1 0.99 14.10 1 1 1 1 a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b C. pneumoniae ATCC VR-1310 concentration as calculated from probit analysis. c Replicates calculated in Microsoft Excel spreadsheet from the following equation: probability (at least 1 positive in n replicates) 5 1 2 (1 2 probit)n. on M arch 15, 2015 by S U N Y H S C B M E D IC A L R E S E A R C H LIB R A R Y http://jcm .asm .org/ D ow nloaded from at lower concentrations of target DNA, and at these concentrations replicates of 3, 5, or 10 would identify increasingly more specimens as positive compared with a single PCR. ..

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS). .. The SPSS statistical program generated the probit (predicted proportion positive) versus the C. pneumoniae concentration with 95% confidence intervals (CI) shown in Table .

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Conversely, at >5 IFU/ml, a single PCR determination is 95% sensitive for detecting C. pneumoniae , and replicates would not increase sensitivity further. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Probit a C. pneumoniae concn (IFU/ml) b No. of replicates needed to achieve sensitivity of c : 50% 80% 90% 95% 0.01 0.01 69 161 230 299 0.05 0.03 14 32 45 59 0.10 0.05 7 16 22 29 0.20 0.09 4 8 11 14 0.30 0.15 2 5 7 9 0.40 0.24 2 4 5 6 0.50 0.35 1 3 4 5 0.60 0.53 1 2 3 4 0.70 0.81 1 2 2 3 0.80 1.34 1 1 2 2 0.90 2.70 1 1 1 2 0.95 4.79 1 1 1 1 0.99 14.10 1 1 1 1 Open in a separate window a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b C. pneumoniae ATCC VR-1310 concentration as calculated from probit analysis. c Replicates calculated in Microsoft Excel spreadsheet from the following equation: probability (at least 1 positive in n replicates) = 1 − (1 − probit) n . Relationship between the predicted number of PCR replicates needed to achieve various test sensitivities and C. pneumoniae concentrations In Fig. , the relationship between increasing number of replicates and overall test sensitivity is presented in graphical form, with the curve for the 10 replicate PCR curve on the far left and that for a single PCR on the far right. ..

    Nested PCR:

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. Conversely, at a concentration of 5 IFU/ml or greater, 9 of 10 replicates would be expected to be positive with either PCR, and a single PCR determination would be positive 90 to 95% of the time. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Probit a C. pneumoniae IFU/ml (95% CI) Non-nested PCR b Nested PCR c 0.01 0.02 (0.01, 0.03) 0.01 (0.003, 0.02) 0.05 0.05 (0.02, 0.08) 0.03 (0.01, 0.05) 0.10 0.08 (0.04, 0.13) 0.05 (0.02, 0.08) 0.15 0.12 (0.07, 0.18) 0.07 (0.04, 0.11) 0.20 0.16 (0.10, 0.24) 0.09 (0.06, 0.14) 0.30 0.27 (0.17, 0.40) 0.15 (0.10, 0.23) 0.40 0.41 (0.28, 0.61) 0.24 (0.16, 0.35) 0.50 0.61 (0.42, 0.92) 0.35 (0.24, 0.52) 0.60 0.92 (0.62, 1.42) 0.53 (0.36, 0.80) 0.70 1.41 (0.94, 2.29) 0.81 (0.55, 1.28) 0.80 2.34 (1.51, 4.08) 1.34 (0.88, 2.23) 0.90 4.69 (2.82, 9.25) 2.70 (1.66, 5.12) 0.95 8.34 (4.68, 18.4) 4.79 (2.77, 10.2) 0.99 24.50 (11.9, 67.8) 14.1 (7.07, 37.3) Open in a separate window a Probit, predicted proportion of replicates positive from probit regression analysis (SPSS), based on 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see the text). b Campbell et al. ( 6 ). c Tong and Sillis ( 23 ). ..

    Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
    Article Snippet: .. At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS). .. The SPSS statistical program generated the probit (predicted proportion positive) versus the C. pneumoniae concentration with 95% confidence intervals (CI) shown in Table .



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